Organic Chemistry
What provides surface for the vapors to cool and condense in a fractionating column?
In column chromatography, the moving phase is?
Column chromatography method used to isolate a single chemical compound from a mixture. Chromatography is able to separate substances based on differential adsorption of compounds to the adsorbent. Compounds move through the column at different rates, allowing them to be separated into fractions.
In column chromatography, the stationary phase, a solid adsorbent, is placed in a vertical glass (usually) column. The mobile phase or eluent is a solvent or a mixture of solvents along with substances to be separated and used to move the compounds through the column.
What provides surface for the vapors to cool and condense in a fractionating column?
A form of partition, adsorption, ion exchange, or affinity chromatography in which one phase is liquid (aqueous) flowing down a column packed with the second phase, a solid is called _______.
The $$R_{f}$$ value of A and B in a mixture determined by TLC in a solvent are $$0.65$$ and $$0.42$$ respectively. If the mixture is separated by column chromatography using the same solvent mixture as a mobile phase, which of the two compounds, A or B will elute first? Explain.
Define the term 'elution' as applied to column chromatography.
What is a fractionating column ? How does it help in the separation of miscible liquids ? Give one example.
Without using column chromatography, how will you separate a mixture of camphor and benzoic acid?
Fill in the blanks : In column chromatography ................. constitutes the stationary phase and the ........... as the molecule phase.
Fill in the blanks : In column chromatography, the .......... absorbed component will elute with the more .......... solvent.
Separation of organic compounds by column chromatography is due to: