Single Choice

In column chromatography, the moving phase is?

AThe substances which are to be separated
BEluent
CAdsorbent
DMixture of eluent and substances to be separated
Correct Answer

Solution

Column chromatography method used to isolate a single chemical compound from a mixture. Chromatography is able to separate substances based on differential adsorption of compounds to the adsorbent. Compounds move through the column at different rates, allowing them to be separated into fractions.

In column chromatography, the stationary phase, a solid adsorbent, is placed in a vertical glass (usually) column. The mobile phase or eluent is a solvent or a mixture of solvents along with substances to be separated and used to move the compounds through the column.


SIMILAR QUESTIONS

Organic Chemistry

What provides surface for the vapors to cool and condense in a fractionating column?

Organic Chemistry

A form of partition, adsorption, ion exchange, or affinity chromatography in which one phase is liquid (aqueous) flowing down a column packed with the second phase, a solid is called _______.

Organic Chemistry

The $$R_{f}$$ value of A and B in a mixture determined by TLC in a solvent are $$0.65$$ and $$0.42$$ respectively. If the mixture is separated by column chromatography using the same solvent mixture as a mobile phase, which of the two compounds, A or B will elute first? Explain.

Organic Chemistry

Define the term 'elution' as applied to column chromatography.

Organic Chemistry

What is a fractionating column ? How does it help in the separation of miscible liquids ? Give one example.

Organic Chemistry

Without using column chromatography, how will you separate a mixture of camphor and benzoic acid?

Organic Chemistry

Fill in the blanks : In column chromatography ................. constitutes the stationary phase and the ........... as the molecule phase.

Organic Chemistry

Fill in the blanks : In column chromatography, the .......... absorbed component will elute with the more .......... solvent.

Organic Chemistry

Separation of organic compounds by column chromatography is due to:

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