Subjective Type

How does one visualise DNA on an agarose gel?

Solution

DNA fragments separate when they are moved towards anode in and electric field. Agarose gel provides the matrix through which DNA fragments separate and move under the influence of electric charge. Separated DNA fragments can be visualized only after staining with ethidium bromide and then by exposure to UV radiation. After staining, DNA fragments appear as bright orange bands.

SIMILAR QUESTIONS

Biotechnology

Having become an expert on gel electrophoresis, you are asked to examine a gel for a colleague. Where would you find the smallest segments of DNA?

Biotechnology

Which of the following steps should be performed by a person in order to visualise the bands of DNA fragments obtained from gel electrophoresis?

Biotechnology

Select the correct option to fill up the blanks. (i) _____ is a natural polymer extracted from ______. (ii) The DNA fragments purified by gel electrophoresis are used in constructing _____ by joining them with ______. (iii) The ligation of alien DNA is carried out at a _____ present in one of the two ____ in a plasmid vector. (iv) _____ enzyme remains active during the high temperature-induced denaturation of ds DNA. DNA fragments are resolved according to their ______ (v) through _____ in agarose gel electrophoresis.

Biotechnology

A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.

Biotechnology

A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?

Contact Details