Biotechnology
What is the source of Eco RI?
In the isolation of DNA, removal of protein and RNA is carried out by enzymes _____ and _________ respectively.
In order to cut the DNA with restriction enzymes, it needs to be in pure form, free from other macromolecules. Since the DNA is enclosed by the membranes, we have to break the cell open to release DNA and other macromolecules like RNA, proteins, polysaccharides, and lipids.
It is obtained by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells) and chitinase (fungus). DNA is intertwined with proteins like histones.
RNA can be removed by treatment with ribonuclease while proteins can be removed by treatment with protease. Other molecules are removed by appropriate treatments and purified DNA ultimately precipitates out after the addition of chilled ethanol.
What is the source of Eco RI?
During isolation of genetic material, the chemical used to precipitate out the purified DNA is
Select the incorrect Statement regarding DNA replication with respect to the template strand
While isolating DNA from bacteria, which of the following enzymes is not used?
Would you choose an exonuclease while producing a recombinant DNA molecule?
What is the significance of adding proteases at the time of isolation of genetic material (DNA).